Bioprocess reducing the solubility of rhizospheric cadmiun

The project proposes an innovative, efficient, and environmentally friendly bioprocess that reduces Cd bioaccumulation. Its scale-up to the territory and training for farmers and interested entrepreneurs to implement it on farms.

Context of the story

The empowerment of the Latin American market for domestic fine aroma cocoa has been limited by high cadmium (Cd) content in almonds. This project proposes an innovative, efficient, and climate-smart bioprocess to reduce Cd bioaccumulation, utilizing mycorrhizal symbiosis and saprobic fungi, accompanied by a protocol for application in the field through an innovative scaling procedure that proposes an adjustment between TRL 4 and TRL 6. All this will be carried out through Technical Cooperation between scientists, farmers, entrepreneurs, and the government, which will impact a total of 3,150 direct beneficiaries, 3,000 indirect beneficiaries involved in the cocoa value chain, thesis students, entrepreneurs, and scientific publications.

The aim of the project is to reduce the cocoa bean Cd concentration to values below 0.8 mg kg-1 dry weight in the cocoa plantations where this biotechnology will be installed.

The implemented initiative

The bioprocess will involve the isolation of native fungi from cocoa plantations, capable of reducing the root and aerial bioaccumulation coefficient of cadmium; validation in bioreactors and scaling up to TRLs 6 and 7 for family farmers' farms. Main Objective: To reduce Cd concentrations to values ??less than 0.8 mg kg-1 in cocoa beans through a bioprocess, accompanied by a protocol for application in the field. Specific Objectives: 1) Create an Entrepreneurs' Laboratory; 2) Formulate the bioprocess in bioreactors (TRL4); 3) Implement and scale up to TRL levels 6-7; and 4) Manage knowledge, communication, and transfer. Scope: 3,150 direct beneficiaries, 3,000 indirect beneficiaries, thesis students, entrepreneurs, and other regions with similar problems.

To generate a bioprocess based on a fungal consortium that will reduce the Cd content in cocoa beans.

The technological solution

Este proyecto pretende disminuir la concentración de cadmio en las almendras de cacao a valores menores a 0,8 mg kg-1 de materia seca a través del manejo de la microbiota y la validación a diversos TRL hasta llegar a territorio. Se aislarán hongos saprobios y micorrícicos que disminuyan la biodisponibilidad del cadmio para la planta de cacao, mediante estrategias fúngicas como exclusión, translocación y solubilidad. Este bioproceso será validado en biorreactores ajustando las condiciones físico-hidráulico-químico-biológico para ser reproducible a TRL 6 y así obtener un protocolo de escalamiento a TRL 7 para ser aplicado en territorio.
Esta tecnología se hará efectiva en las zonas de Calceta, Ecuador y en la Península de Paria Venezuela, y podrá aplicarse en otras regiones con problemas semejantes.
Esto permitirá mejorar las condiciones de vida de la región, incrementando los ingresos de los beneficiarios, poniendo énfasis en la promoción de las mujeres involucradas.

“It’s the start of a new era, when people are finally ready to embrace the microbial world”

Ed Yong, science journalist

Participating countries

Type of project

Results

975 / 5.000
The projected figures for the technical indicators were exceeded in terms of beneficiaries, technological solutions, networks formed, scientific production, and theses involved. The project's impact was greater at the scientific, environmental, social-educational, and gender levels than at the socioeconomic levels. The results are detailed in the indicator format: Publications: 10 articles in scientific journals, 10 book chapters, 21 conference presentations. 931 people trained, of whom 205 are women. Total beneficiaries: 3,452 people, of whom 1,646 are women.
Theses: 1 doctoral theses defended, 2 doctoral theses in progress, 1 undergraduate theses defended, 1 undergraduate theses in progress. 3 Technological solutions and innovations: linked to arbuscular fungi: mycorrhizal symbiosis with different behavior: flower-excluding, phytostabilizing, cadmium hyperaccumulating; linked to saprobic fungi; and linked to scaling and conservation of conditions.